Subgingival plaque samples were obtained from the three pockets with the deepest probing depths. Two sterile paper points were inserted simultaneously into the periodontal pockets.

tively characterize the total culturable subgingival-plaque flora of one dog with ligatures and one dog without. Thus, at numer- ous time points, plaque samples

Two sterile paper points were inserted simultaneously into the periodontal pockets. 2018-09-19 · Subgingival plaque sampling. Microbial sampling was undertaken at the three deepest interproximal sites of non-adjacent teeth in every patient. Sampling in three out of four quadrants was conducted wherever possible. In 25 subjects, one healthy, gingivitis and periodontitis site was sampled for GCF using a strip followed by subgingival plaque sampling with a curette. Bacterial assays indicated that GCF strips removed significant numbers of bacteria when placed intracrevicularly for 5 s. Microbial analysis of subgingival plaque samples compared to that of whole saliva in patients with periodontitis DSpace Repository JavaScript is disabled for your browser.

Significantly lower mean proportions of spirochetes and motile rods and higher proportions of non‐motile organisms were obtained with subgingival washing compared with curette sampling. The presence of specific bacteria in subgingival plaque has been used as an indicator of active periodontal disease. The technique of subgingival sampling may conjecturally influence the identification and enumeration of microorganisms reported. In this study, paper point sampling and scaler sampling are compared. Have minimum 6 sampling sites with bleeding and pocket depth ≥2mm but not deeper than 4mm. Exclusion Criteria: Have had a dental prophylaxis within 2 weeks of plaque sampling visits; Have taken antibiotics or used anti-gingivitis / anti-bacterial oral care products such as chlorhexidine or Listerine within 2 weeks of plaque sampling visits; Subgingival plaque samples within the deepest pocket of each tooth were taken and analysed by real-time polymerase chain reaction (PCR) for Actinobacillus actinomycetemcomitans (AA), Porphyromonas 1994-08-01 · A subgingival plaque sample was then obtained using a Gracey curette machined to a blade width of 0.5 mm to facilitate access to the subgingal area. The subgingival plaque from each site was collected by placing the curette at the apical extent of the gingival crevice and drawing it coronally with slight pressure against the tooth surface.

53 samples 53 crises 53 schedules 53 listings 53 celebrations 53 blockades 53 warm-up 73 plaque 73 vengeance 73 hegemony 73 bombshell 73 armistice 121 0925 121 Avance 121 subgingival 121 4.3-percent 121 airport-to-airport i en in vitro multispecies oral biofilm efterliknande subgingival dental plaque hade The samples were then concentrated using a Speedvac (Thermo Savant Subgingival depuraion, vill först at den behandla munhygienen men får ha i Removal of calculus and bacterial plaque will eliminate or markedly reduce the Bakteriell plack från subgingivalskador provades vid baslinjen och denna For microbiological sampling, the deepest non-furcated site in each quadrant was After supragingival plaque removal, 2 paper points (Henry Schein, Almere, The personal norms and support for environmental policy instruments, testing this on a sample of over Swedish students.

Effect of gingival fluid collection on subgingival plaque sampling. Author links open overlay panel B. Mullally a L. Wolff ∗ N. Hardie ∗ D. Aeppli ∗ B. Pihlstrom ∗

After supragingival plaque was carefully removed and sampling sites were isolated with cotton rolls and air-dried, a subgingival plaque sample was collected with 2 sterile paper points per site. Plaque sampling for DNA test was performed at the baseline, 1 week after professional tooth cleaning, and after 3 weeks of either miswak or toothbrush use. Identification and quantification of microbial species were performed by the checkerboard method, using whole genomic, digoxigenin-labelled DNA probes. (2017) Okada et al.

av S Edwardsson · Citerat av 3 — man herpes virus 7 from saliva samples. J Med Virol 1999 Ericson RE, Lau CN et al. Bacterial diversity in human subgingival plaque.

morphotypes in subgingival samples in cases of periodontal health, gingivitis, and Supragingival plaque typically demonstrates a stratified organization of a The whole blood sampling handbook.
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Significantly lower mean proportions of spirochetes and motile rods and higher proportions of non-motile organisms were obtained with subgingival washing compared with curette sampling. Background: The purpose of this study was to compare the results of microbiological RNA‐probe analysis after subgingival plaque sampling applying two different strategies. Methods: In 220 patients, clinical examinations were obtained prior to commencement of therapy for aggressive or generalized severe chronic periodontitis (n = 113), after combined mechanical and antibiotic anti‐infective subgingival plaque sampling strategy and analysis strategy with oligonucleotide probes Bernadette Pretzl, Jule Paul, Diana M. Krigar, Lorenz Uhlmann, Peter Eickholz & Bettina Dannewitz Comparison of Two Different Sampling Methods for Subgingival Plaque: Subgingival Paper Points or Mouthrinse Sample? Katrin Nickles.

Of the sampling of all assessed sites, the highest probability for simultaneously detecting the tested pathogens was found in respect to the combination of supra- and subgingival plaque samples 1994-08-01 Conclusion: Salivary analysis might be discussed as a potential alternative to subgingival plaque sampling for microbiologic analysis in both AgP and CP. Discover the world's research. Subgingival plaque sampling and clinical recording (at baseline) and scaling and root planing was performed. Two weeks later the selected periodontal sites were submitted to one of the following treatments: Irrigation with a hydroalcoholic solution of propolis extract twice a week for 2 weeks (group A); irrigation with a placebo twice a week for 2 weeks (group B); or no additional treatment Before and immediately after treatment, subgingival plaque samples were taken from interdental sites with 3 to 5 mm probing depth (PD) at 2 test teeth and 2 positive control teeth.
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The whole blood sampling handbook. Find a Lower Price? We'll Refund the Prevotella heparinolytica. Subgingival dental plaque. Prevotella histicola. Human

Tooth brushing twice daily to reduce oral debris and dental plaque remains the gingivitis may serve as a portal for infection and further result in subgingival. Microbial analysis of subgingival plaque samples compared to that of whole saliva in patients with periodontitis. J Periodontol 2014 Jun;.

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2.4. Subgingival Plaque Sampling and Quantitative Bacterial Assay. After removing the supragingival plaque, a subgingival plaque sample was taken by inserting 2 sterile number 40 paper points (Zipperer Absorbent Paper Points, VDW GmbH, Munich, Germany) consecutively into the periodontal pocket for 10 s at each of the selected sites.

If “contamination” of the sample is relatively high, human DNA may dominate sequencing efforts resulting in a low coverage of microbial DNA, hampering analysis of the microbiome. Subgingival sampling Supragingival plaque should be removed with cotton pellets or periodontal scalers to secure noncontaminated subgingival specimens.

2015-07-21

Microbial analysis of subgingival plaque samples compared to that of whole saliva in patients with periodontitis DSpace Repository JavaScript is disabled for your browser.

The presence of specific bacteria in subgingival plaque has been used as an indicator of active periodontal disease. Comparison of Two Different Sampling Methods for Subgingival Plaque: Subgingival Paper Points or Mouthrinse Sample? Nickles K(1), Scharf S(1), Röllke L(1), Dannewitz B(1)(2), Eickholz P(1). Author information: (1)Department of Periodontology, Center for Dentistry and Oral Medicine (Carolinum), Goethe University, Frankfurt, Germany. A Study to Evaluate Sampling Methods for Subgingival Plaque.